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Epizyme Inc rapid membrane transfer buffer
Rapid Membrane Transfer Buffer, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transfer+buffer/buffer+transfer/pmc13254979-127-0-5
Average 86 stars, based on 1 article reviews
rapid membrane transfer buffer - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Membrane:

Article Title: Modified Shenqi Dihuang Decoction inhibits prostate cancer metastasis by disrupting TCA cycle energy metabolism via NF-kB/p65-mediated OGDH regulation.
Article Snippet: Background: Prostate cancer (PCa) is a significant malignancy in men, particularly challenging in the metastatic stage due to poor prognosis and limited treatment efficacy.. Traditional Chinese Medicine, particularly Modified Shenqi Dihuang Decoction (MSDD), has demonstrated promise in inhibiting PCa metastasis, although its mechanisms remain unclear.. Methods: The efficacy of MSDD was evaluated using migration assays and a nude mouse model. Metabolomics was employed to identify the biological processes affected by MSDD.

CCK-8 Assay:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

RNA Extraction:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

AST Assay:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

SYBR Green Assay:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Real-time Polymerase Chain Reaction:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

SDS Page:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Bicinchoninic Acid Protein Assay:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Polyacrylamide Gel Electrophoresis:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Western Blot:

Article Title: Potential hepatoprotective effects of Cistanche deserticola Y.C. Ma : Integrated phytochemical analysis using UPLC-Q-TOF-MS/MS, target network analysis, and experimental assessment
Article Snippet: .. The materials obtained are as follows: 1640 medium and fetal bovine serum (FBS) (Gibco, United States); penicillin–streptomycin liquid (Bioharp, Hefei, China); ECH (Chengdu Must Bio-technology Co., Ltd.; Chengdu, China); LPS (Meilunbio, Dalian, China); FITC-LPS (Sigma, United States); CCK-8 Kit and Simply P Total RNA Extraction Kit (Bioss, Beijing, China); MCP-1, IL-1β, IL-6, TNF-α, and NO Elisa Kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China); ALT and AST reagents (URIT Medical Electronic Co., Ltd., Guilin, China); StarLighter SYBR Green qPCR MIX and StarLighter Script RT all-in-one MIX (Beijing Foreverstar Biotech Co., Ltd., Beijing, China); PMSF, RIPA tissue/cell lysate (Beijing Solarbio Science and Technology Co., Ltd., Beijing, China); SDS-PAGE Sample Loading Buffer and BCA Protein Assay Kit (Beyotime Biotechnology Company, Beijing, China); PAGE Gel Fast Preparation Kit, Tris-glycine/SDS running buffer, transfer buffer, and TBST (Shanghai Epizyme Biomedical Technology Co., Ltd., Shanghai, China); primary antibody and secondary antibody dilution for Western blot (Absin, Shanghai, China). ..

Electrophoresis:

Article Title: Polymer-assisted PD-L1 degradation and targeted photodynamic therapy synergize to suppress immunodeficient tumors
Article Snippet: TNF α , IL-6, IFN- γ ELISA kits, and cell apoptosis assay kits were purchased from MultiSciences Technology Co., Ltd. (Shanghai China). .. Electrophoresis buffer and transfer buffer were purchased from Epizyme Biomedical Technology Co., Ltd. (Shanghai China). .. The serum was purchased from Gibco (Brazil South America).



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